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A human cDNA library was screened for sequences homologous to the erbA gene of avian erythroblastosis virus (AEV). One such clone, cHerbA-1, was used to map the chromosomal location of highly homologous human sequences that were found to be present on chromosome 17 as judged by Southern blot screening of a panel of mouse-human hybrid cell lines segregating human chromosomes. cHerbA-1 was hybridized in situ to metaphase chromosomes from a normal male subject and from a female patient with an acute promyelocytic leukemia (APL) having the typical t(15;17) translocation. The results localized the cellular c-erbA sequences on chromosome 17 to the q21-q24 region of normal chromosomes and indicated that the c-erbA sequences remained on the 17q- chromosome in the APL cells, suggesting that they could be assigned to the 17(q21-q22) region. For additional data, we hybridized human neoplastic cells derived from a poorly differentiated acute leukemia carrying a t(17;21) translocation with thymidine kinase (TK)-deficient LMTK- mouse cells. A resulting hybrid, containing only the 21q+ chromosome, did not have human c-erbA sequences. Since the breakpoint on 17q in this translocation was similar to that in the APL t(15;17) translocation, this supported the assignment of c-erbA to the q21-q22 region of chromosome 17. The apparent close proximity of the c-erbA sequences to the chromosomal breakpoints in these two leukemias suggests a possible role for this oncogene homologue in the development of these neoplasms.
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
July 1985,
Annals of human genetics,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
September 1990,
Science (New York, N.Y.),
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
July 1987,
Blood,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
April 1982,
Cancer genetics and cytogenetics,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
February 1989,
Genomics,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
January 1987,
Acta haematologica,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
January 1988,
Cancer genetics and cytogenetics,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
December 1987,
Leukemia,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
May 1977,
Archives of internal medicine,
A I Dayton, and
J R Selden, and
G Laws, and
D J Dorney, and
J Finan, and
P Tripputi, and
B S Emanuel, and
G Rovera, and
P C Nowell, and
C M Croce
May 1987,
Leukemia,
