Biomaterial Database

Protein biosynthesis during spiny dogfish (Squalus acanthias) enameloid formation. 1984

E E Graham

Little is known about protein biosynthesis during enameloid formation. The purpose of this study was to characterize protein biosynthesis during selachian amelogenesis. Experiments were conducted on intact shark tooth organs, surgically removed from the spiny dogfish, Squalus acanthias. These organs were cultured in medium labelled with [35S]-methionine during time periods ranging from 1 to 6 h in pulse/chase experiments. The proteins were extracted at the conclusion of the experiments for analysis by gel electrophoresis and fluorography. In addition, tooth organs were fixed and examined by light microscopic autoradiography. Through this outlined experimental strategy, protein biosynthesis during amelogenesis was examined. The findings suggest the ameloblasts, at early bell stage of tooth development, are biosynthetically more active than the incipient odontoblasts; and they are secreting two polypeptides, 55 and 43 kd, into the developing tooth extracellular matrix.

UI	MeSH Term	Description	Entries
D009804	Odontoblasts	The mesenchymal cells which line the DENTAL PULP CAVITY and produce DENTIN. They have a columnar morphology in the coronal pulp but are cuboidal in the root pulp, or when adjacent to tertiary dentin.	Odontoblast
D009924	Organ Culture Techniques	A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)	Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D003746	Dental Enamel Proteins	The proteins that are part of the dental enamel matrix.	Enamel Proteins, Dental,Proteins, Dental Enamel
D004284	Dogfish	Sharks of the family Squalidae, also called dogfish sharks. They comprise at least eight genera and 44 species. Their LIVER is valued for its oil and its flesh is often made into fertilizer.	Squalidae,Dogfishes
D000565	Ameloblasts	Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)	Ameloblast
D000566	Amelogenesis	The elaboration of dental enamel by ameloblasts, beginning with its participation in the formation of the dentino-enamel junction to the production of the matrix for the enamel prisms and interprismatic substance. (Jablonski, Dictionary of Dentistry, 1992).	Amelogeneses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012754	Sharks	A group of elongate elasmobranchs. Sharks are mostly marine fish, with certain species large and voracious.	Shark