Biomaterial Database

Clean-up procedure for the extraction of soil samples in the determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin. 1978

I Camoni, and A Di Muccio, and D Pontecorvo, and L Vergori

This paper describes the method which was developed in relation to analytical work connected with microbial and physico-chemical degradation experiments on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Soil samples are best extracted with methanol plus methylene chloride. Microbial preparations are extracted with light petroleum after boiling with methanolic potassium hydroxide. The clean-up consists of a sulphuric acid treatment and chromatography on a multilayer column (Celite + H2SO4/silica gel) followed by alumina column chromatography. The clean-up procedure proved to be suitable for soil samples and microbial preparations even when large quantities of organic matter (hydrocarbons, oils, surfactants) were present.

UI	MeSH Term	Description	Entries
D007220	Industrial Waste	Worthless, damaged, defective, superfluous or effluent material from industrial operations.	Waste, Industrial,Industrial Wastes,Wastes, Industrial
D007558	Italy	A country in southern Europe, a peninsula extending into the central Mediterranean Sea, northeast of Tunisia. The capital is Rome.	Sardinia
D004147	Dioxins	A family of compounds that contain the 1,4-dioxin structure. Many specific dioxin derivatives are listed as CARCINOGENS; TERATOGENS; or MUTAGENS.	Dioxin
D000072317	Polychlorinated Dibenzodioxins	Dibenzodioxin derivatives that contain multiple chloride atoms bound to the benzene ring structures.	TCDD,Tetrachlorodibenzodioxin,2,3,7,8-Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo-p-dioxins,Dibenzo(b,e)(1,4)dioxin, 2,3,7,8-tetrachloro-,PCDD,Polychlorinated Dibenzo-p-dioxins,Polychlorinated Dibenzodioxin,Polychlorodibenzo-4-dioxin,Polychlorodibenzo-p-dioxin,Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo p dioxins,Dibenzo-p-dioxins, Chlorinated,Dibenzo-p-dioxins, Polychlorinated,Dibenzodioxin, Polychlorinated,Dibenzodioxins, Polychlorinated,Polychlorinated Dibenzo p dioxins,Polychlorodibenzo 4 dioxin,Polychlorodibenzo p dioxin,Tetrachlorodibenzo p dioxin
D001711	Biotransformation	The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
D012988	Soil Microbiology	The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.	Microbiology, Soil
D012989	Soil Pollutants	Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.	Soil Pollutant,Pollutant, Soil,Pollutants, Soil