Biomaterial Database

Prostaglandin E2 production by human bone marrow cells: a comparison with peripheral blood mononuclear cells. 1984

Y Nakayama, and T Shindo, and T Akihama, and A B Miura

The amount of prostaglandin E2 (PGE2) in the supernatant of human bone marrow cell cultures was measured by radioimmunoassay. Bone marrow cells were obtained from adult patients without hematological malignancies. Marrow mononuclear cells (BMMNC) (0.5 X 10(6)) and marrow nonadherent cells (BMNAC) (0.5 X 10(6)) were cultured at 37 degrees C in 5% CO2 in 0.5 ml RPMI 1640 supplemented with L-glutamine, 10% fetal calf serum and 10 mM Hepes buffer. The levels of PGE2 in the supernatant of BMMNC after 24 hr and 48 hr incubation were 3,000 +/- 2,320 pg/ml (n = 10, mean +/- S.D.) and 4,470 +/- 3,510 pg/ml (n = 12), respectively. The kinetics of PGE2 production by BMMNC was different from that of peripheral blood mononuclear cells. The level of PGE2 in the supernatant of BMNAC after 48 hr incubation was only 1/18 when compared to that of BMMNC. These data suggest that the major source of PGE2 in the supernatant of marrow cell cultures may be bone marrow mononuclear phagocytes and that admixed blood mononuclear cells in BMMNC may produce only a small amount of PGE2.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007962	Leukocytes	White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).	Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D011458	Prostaglandins E	(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.	PGE
D001853	Bone Marrow	The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.	Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006410	Hematopoiesis	The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).	Hematopoiesis, Medullary,Haematopoiesis,Medullary Hematopoiesis
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015232	Dinoprostone	The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.	PGE2,PGE2alpha,Prostaglandin E2,Prostaglandin E2alpha,PGE2 alpha,Prepidil Gel,Prostaglandin E2 alpha,Prostenon,E2 alpha, Prostaglandin,E2, Prostaglandin,E2alpha, Prostaglandin,Gel, Prepidil,alpha, PGE2,alpha, Prostaglandin E2