-transparent.png){kind=logo}
Infective forms of Trypanosoma brucei derived from the midgut of Glossina morsitans, have been propagated in vitro for 61 days on a bovine embryonic spleen (BESP) feeder layer using RPMI 1640 medium. It was reproducibly shown that only parasites cultured from the midgut of tsetse flies 12-14 hr after feeding on infected animals could be established in vitro. Cultures thus established were infective to rats and tsetse flies. Only midgut vector types of the parasites were identified by light and electron microscopic techniques.
Related Publications
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
January 1992,
Parasitology research,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
February 1981,
Parasitology,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
January 1982,
Zeitschrift fur Parasitenkunde (Berlin, Germany),
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
September 1987,
Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ),
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
May 1989,
Acta tropica,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
July 1997,
Medical and veterinary entomology,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
January 1980,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
December 1979,
Acta tropica,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
August 1989,
Annals of tropical medicine and parasitology,
M Nyindo, and
N Darji, and
J S Kaminjolo, and
M Chimtawi, and
N Patel, and
P Onyango
January 2004,
Veterinary parasitology,
