Biomaterial Database

Isoelectric focusing of beta-lactamases on cellulose acetate membranes. 1983

A Eley, and J Ambler, and D Greenwood

Cellulose acetate membranes treated with boron trifluoride in methanol were found to provide suitable support material for isoelectric focusing of beta-lactamases; the method used is described. A linear pH gradient was obtained and the application of visible pI markers allowed the pH gradient to be monitored as focusing proceeded. beta-Lactamases extracted from antibiotic-resistant coliform organisms focused as sharp, straight bands upon visualization with nitrocefin. Results were known within two hours of applying the beta-lactamase extracts. The method allows a very economical use of expensive ampholyte and offers a quick and more simple alternative procedure for the identification of beta-lactamases than the conventional polyacrylamide gel method.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D004588	Electrophoresis, Cellulose Acetate	Electrophoresis in which cellulose acetate is the diffusion medium.	Cellulose Acetate Electrophoreses,Cellulose Acetate Electrophoresis,Electrophoreses, Cellulose Acetate
D004755	Enterobacteriaceae	A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.	Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001618	beta-Lactamases	Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.	beta-Lactamase,beta Lactamase,beta Lactamases