Biomaterial Database

Evidence that active and stable E-rosette forming cells belong to different human T lymphocyte subpopulations. 1983

S C Paris, and L F García

Human T Lymphocytes form spontaneous E-rosettes with sheep erythrocytes. Cells with high affinity E-receptors can be differentiated by their formation of active (aE) or stable (sE)-rosettes; therefore, experiments were performed in order to determine if active and stable E-rosettes are formed by different populations of T lymphocytes. "Enriched" and "depleted" fractions of aE-RFC were separated on Ficoll-Hypaque after fresh peripheral blood lymphocytes were mixed with SRBC under aE-rosette conditions. Enrichment of aE-RFC did not induce a simultaneous increase in the number of sE-RFC. Furthermore, upon incubation with Con A, a significant increase in the percentage of sE-RFC was detected in the aE-RFC "depleted" preparation. Fractionation of lymphocytes which had been cultured with Con A into sE-"enriched" and "depleted" populations again showed that aE-and sE-RFC do not co-separate in to the same fractions. Analysis of T cell-enriched cultures, prepared from peripheral blood lymphocytes either by rosetting with SRBCaet or fractionation on nylon wool columns, which were stimulated with Con A and then fractionated into aE-or sE- "enriched" and "depleted" populations clearly showed that enrichment of aE-RFC results in a decrease in the number of sE-RFC and viceversa. These data strongly support the idea that cells forming active and stable E-rosettes belong to different populations of human T lymphocytes.

UI	MeSH Term	Description	Entries
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012397	Rosette Formation	The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.	Immunocytoadherence,Formation, Rosette,Formations, Rosette,Immunocytoadherences,Rosette Formations
D013601	T-Lymphocytes	Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.	T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte