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A culture system was developed in which human peripheral blood mononuclear cells depleted of monocytes did not proliferate in response to the lectin mitogen phytohemagglutinin (PHA). The response of these cells to PHA was reconstituted with both autologous and heterologous alveolar macrophages. In addition, a partial restoration of PHA responsiveness was seen when the monocyte-depleted cells (MDC) were cultured with supernatants from purified alveolar macrophages, indicating the presence of Interleukin-1 (IL-1) activity in these supernatants. This is the first evidence suggesting the production of IL-1 by tissue macrophages from normal human subjects. In contrast to previous studies of IL-1 production by blood macrophages, it was found to be unnecessary to stimulate the alveolar macrophages in vitro to generate soluble factor activity. It was also found that alveolar macrophages produced active supernatants after only 2 hr in culture, whereas previous studies have indicated that blood monocytes must be cultured for at least 24 hr before active supernatants are produced. These differences may provide evidence that macrophages within the alveolar spaces are in an activated state.
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
June 2005,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
October 1992,
The Journal of infectious diseases,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
August 1989,
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G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
December 1991,
Neuropeptides,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
November 1998,
Leukemia research,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
February 1999,
American journal of respiratory cell and molecular biology,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
January 1986,
Nihon Kyobu Shikkan Gakkai zasshi,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
February 1989,
The American journal of physiology,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
May 1989,
The American journal of physiology,
G A Koretzky, and
J A Elias, and
S L Kay, and
M D Rossman, and
P C Nowell, and
R P Daniele
February 1993,
Nihon Kyobu Shikkan Gakkai zasshi,
