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Media conditioned with 8 cell lines were assayed for stimulating and inhibiting activities on granulocyte-macrophage colony formation from bone marrow cells in soft agar (CFUc). Conditioned media from 3 human bladder cancer cell lines (EJ, T24 and KK47) and a rat bladder cancer cell line (BC50-TC) showed marked inhibition of mouse CFUc. Conditioned medium from normal human bladder cells showed a weak colony-stimulating activity, and only a slight inhibition when added to CFUc culture. Conditioned media from mouse or rat fibroblast cell lines (BLP and 3Y1) showed no colony-inhibiting activity, and the conditioned media from human lymphoblastoid cell line (Raji) and human epithelia-like cell line from amniotic membrane (FL) showed moderate inhibitory activity. No colony-stimulating activity was detected in any of the cell lines tested. EJ-conditioned medium inhibited human CFUc, but not erythroid progenitors. Colony-inhibiting activity in EJ-conditioned medium appears not to be prostaglandins; it needs more than 3 hr to inhibit CFUc, is stable at 56 degrees for 30 min but not at 75 degrees for 20 min, and is susceptible to proteolytic enzymes.
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
October 1978,
British journal of haematology,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
October 1988,
Clinical immunology and immunopathology,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
August 1980,
Biochemistry,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
June 1979,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
September 1975,
Journal of the Reticuloendothelial Society,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
August 1989,
Experimental hematology,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
November 1980,
Vnitrni lekarstvi,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
January 1982,
Stem cells,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
January 1988,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
J Fujita, and
K Oishi, and
O Yoshida, and
T Kamamoto, and
T Miyanomae, and
K J Mori
January 2002,
Journal of experimental therapeutics & oncology,
