BIOMAT Database Logo BIOMAT Database Logo

Induction of interleukin 1 messenger RNA and translation in oocytes. 1984

J J Windle, and H S Shin, and J F Morrow

Macrophages and P388D1 murine cultured cells produce interleukin 1 (lymphocyte-activating factor) when stimulated with lipopolysaccharide or other agents. Poly A+ RNA extracted from either type of stimulated cells and injected into Xenopus oocytes causes synthesis of material with the biologic and biochemical properties of interleukin 1. It potentiates lectin-mediated thymocyte proliferation, and it has the molecular dimensions of interleukin 1, as determined by gel exclusion chromatography. RNA from either cell type causes the synthesis of interleukin 1 with an isoelectric point of 4.8 to 5.0. RNA prepared from unstimulated macrophages or P388D1 cells does not cause interleukin 1 production by oocytes. We conclude that the amount of interleukin 1 mRNA increases greatly after stimulation of either cell type, and oocytes carry out any modifications of the polypeptide necessary for activity. The kinetics of interleukin 1 mRNA accumulation and of interleukin 1 production by macrophages and P388D1 cells are compared.

UI MeSH Term Description Entries
D007375 Interleukin-1 A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation. IL-1,Lymphocyte-Activating Factor,Epidermal Cell Derived Thymocyte-Activating Factor,Interleukin I,Macrophage Cell Factor,T Helper Factor,Epidermal Cell Derived Thymocyte Activating Factor,Interleukin 1,Lymphocyte Activating Factor
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008262 Macrophage Activation The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants. Activation, Macrophage,Activations, Macrophage,Macrophage Activations
D008297 Male Males
D008809 Mice, Inbred C3H An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research. Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D011650 Pulmonary Alveoli Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place. Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D005260 Female Females

Related Publications

J J Windle, and H S Shin, and J F Morrow
Translation of messenger RNA in injected frog oocytes.
January 1987, Methods in enzymology,
J J Windle, and H S Shin, and J F Morrow
Translation of human immune interferon messenger RNA in Xenopus laevis oocytes.
May 1981, Biochemical and biophysical research communications,
J J Windle, and H S Shin, and J F Morrow
Translation of the messenger RNA for rabbit uteroglobin in Xenopus oocytes.
November 1975, FEBS letters,
J J Windle, and H S Shin, and J F Morrow
Cell-free translation of messenger RNA from oocytes of Xenopus laevis.
June 1976, Developmental biology,
J J Windle, and H S Shin, and J F Morrow
The translation of messenger RNA injected in living oocytes of Xenopus laevis.
January 1973, Acta endocrinologica. Supplementum,
J J Windle, and H S Shin, and J F Morrow
Expression of cloned genes and translation of messenger RNA in microinjected Xenopus oocytes.
January 1990, The International journal of biochemistry,
J J Windle, and H S Shin, and J F Morrow
Translation of biologically active messenger RNA from human placenta in Xenopus oocytes.
March 1979, European journal of biochemistry,
J J Windle, and H S Shin, and J F Morrow
Interleukin-1 receptor-like proteins synthesized by translation, in vitro, of immunopurified polysomal messenger RNA.
November 1988, The Journal of biological chemistry,
J J Windle, and H S Shin, and J F Morrow
Translation of messenger RNA specific for tyrosine aminotransferase in oocytes of Xenopus laevis.
September 1976, Biochemical and biophysical research communications,
J J Windle, and H S Shin, and J F Morrow
[Translation of human fibroblast interferon messenger RNA in Tilapiamosaiabica peters oocytes (author's transl)].
March 1980, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
Copied contents to your clipboard!