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Rat osteosarcoma cells respond to 1,25-dihydroxyvitamin D3 with a 6- to 10-fold increase in the secretion of citric acid. The time required to attain a half-maximal response is 12 h, a time course which is consistent with the postulated steroidal hormone action of this vitamin D metabolite. The citrate response is achieved by physiological concentrations of 1,25-dihydroxyvitamin D3, with half of the maximal response at a vitamin concentration of 0.03 ng/ml. Both the time course and the dose dependence of the citrate response closely parallel the previously reported stimulation of bone Gla protein synthesis by 1,25-dihydroxyvitamin D3 in these cells. Citrate and bone Gla protein bind avidly to bone mineral and are numerically the most abundant organic acid and protein in bone. The parallel secretion of both in 1,25-dihydroxyvitamin D3-treated osteoblastic cells suggests that they may act in tandem to mediate an action of this vitamin D metabolite on the mineral phase of bone.
P A Price, and
M K Williamson, and
S A Sloper
May 1994,
Molecular and cellular endocrinology,
P A Price, and
M K Williamson, and
S A Sloper
December 1980,
The Journal of biological chemistry,
P A Price, and
M K Williamson, and
S A Sloper
July 1984,
The Journal of biological chemistry,
P A Price, and
M K Williamson, and
S A Sloper
August 1989,
Archives of biochemistry and biophysics,
P A Price, and
M K Williamson, and
S A Sloper
October 1985,
Nutrition reviews,
P A Price, and
M K Williamson, and
S A Sloper
May 1981,
Diabetes,
P A Price, and
M K Williamson, and
S A Sloper
May 1998,
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
P A Price, and
M K Williamson, and
S A Sloper
November 1985,
Endocrinology,
P A Price, and
M K Williamson, and
S A Sloper
March 1987,
Acta endocrinologica,
P A Price, and
M K Williamson, and
S A Sloper
April 1987,
The Journal of biological chemistry,
