Biomaterial Database

[3H]UTP incorporation in permeabilized mouse L-cells is inhibited by antibodies directed against a structural nuclear protein. 1984

F A Baglia, and G G Maul

Newly transcribed RNA is apparently attached to a nuclear structural framework whose main proteins are lamins A, C and B. Whether this attachment is fortuitous or has some functional importance was tested by measuring [3H]UTP incorporation into RNA of permeabilized mouse L-cells after incubation with antibodies against these polypeptides. Antibodies against only lamin B and not against lamins A, C inhibited low concentration alpha-amanitin-sensitive RNA transcription. Lamin B antibodies inhibited only in an assay system with whole nuclei and not in a soluble assay system. Anti-lamin B therefore does not block the actual transcriptional machinery, but rather seems to crosslink the structural framework on which transcription takes place in the intact nucleus, inhibiting dynamic changes essential to RNA synthesis.

UI	MeSH Term	Description	Entries
D007739	L Cells	A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.	Earle's Strain L Cells,L Cell Line,L Cells (Cell Line),L-Cell Line,L-Cells,L-Cells, Cell Line,L929 Cell Line,L929 Cells,NCTC Clone 929 Cells,NCTC Clone 929 of Strain L Cells,Strain L Cells,Cell Line L-Cell,Cell Line L-Cells,Cell Line, L,Cell Line, L929,Cell Lines, L,Cell, L,Cell, L (Cell Line),Cell, L929,Cell, Strain L,Cells, L,Cells, L (Cell Line),Cells, L929,Cells, Strain L,L Cell,L Cell (Cell Line),L Cell Lines,L Cell, Strain,L Cells, Cell Line,L Cells, Strain,L-Cell,L-Cell Lines,L-Cell, Cell Line,L929 Cell,Strain L Cell
D009698	Nucleoproteins	Proteins conjugated with nucleic acids.	Nucleoprotein
D002463	Cell Membrane Permeability	A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.	Permeability, Cell Membrane
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000974	Antibodies, Antinuclear	Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.	Anti-DNA Antibodies,Antibodies, Anti-DNA,Antinuclear Antibodies,Antinuclear Autoantibodies,Antinuclear Autoantibody,Antinuclear Factors,Antinuclear Antibody,Antinuclear Factor,Anti DNA Antibodies,Antibody, Antinuclear,Autoantibody, Antinuclear,Factor, Antinuclear
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D034882	Lamins	Nuclear matrix proteins that are structural components of the NUCLEAR LAMINA. They are found in most multicellular organisms.	Lamin
D034921	Lamin Type B	A subclass of ubiquitously-expressed lamins having an acidic isoelectric point. They are found to remain bound to NUCLEAR MEMBRANE during MITOSIS.	Lamin B1,Lamin B2,Lamin B3,LMN2 protein,LMNB2 protein,Lamin B,Type B Lamins