Biomaterial Database

The oligosaccharides of human alpha 1-antitrypsin. 1984

A Hercz

Oligosaccharides were prepared from alpha 1-antitrypsin (PiM) and human serum transferrin by hydrazinolysis and then reacetylated with radiolabelled acetic anhydride. Gel permeation chromatography revealed similarities between the two preparations. The antitrypsin oligosaccharides were resolved into 76% concanavalin A binding and 24% nonbinding sugar chains by lectin affinity chromatography; both fractions were further resolved by preparative paper electrophoresis. Small amounts of neutral oligosaccharides, as well as approximately 30% "monosialo" and 67% "disialo" biantennary oligosaccharides, were identified in the lectin-binding pool. The disialo oligosaccharide fraction was susceptible to sequential degradation with exoglycosidases. Upon incubation of the monosialo biantennary oligosaccharide fraction with CMP-[14C]sialic acid and a rat liver Golgi preparation as a source of transferase, a new doubly labelled peak was formed that eluted from the gel permeation chromatography column in the same volume as the disialo biantennary oligosaccharide fraction. The paper electrophoretogram of the lectin-nonbinding oligosaccharides revealed the presence of limited amounts of neutral oligosaccharides and oligosaccharides carrying two and three negative charges. The lectin-nonbinding oligosaccharide fraction with two charges was tentatively identified as disialo biantennary oligosaccharide containing a bisecting N-acetylglucosamine residue, and the fraction with three negative charges was tentatively identified as trisialo triantennary oligosaccharide.

UI	MeSH Term	Description	Entries
D009844	Oligosaccharides	Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.	Oligosaccharide
D002240	Carbohydrate Sequence	The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.	Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000089	Acetic Anhydrides	Compounds used extensively as acetylation, oxidation and dehydrating agents and in the modification of proteins and enzymes.	Anhydrides, Acetic
D000107	Acetylation	Formation of an acetyl derivative. (Stedman, 25th ed)	Acetylations
D000515	alpha 1-Antitrypsin	Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.	Trypsin Inhibitor, alpha 1-Antitrypsin,alpha 1-Protease Inhibitor,alpha 1-Proteinase Inhibitor,A1PI,Prolastin,Serpin A1,Zemaira,alpha 1 Antiprotease,alpha 1-Antiproteinase,1-Antiproteinase, alpha,Antiprotease, alpha 1,Inhibitor, alpha 1-Protease,Inhibitor, alpha 1-Proteinase,Trypsin Inhibitor, alpha 1 Antitrypsin,alpha 1 Antiproteinase,alpha 1 Antitrypsin,alpha 1 Protease Inhibitor,alpha 1 Proteinase Inhibitor
D014168	Transferrin	An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.	Siderophilin,Isotransferrin,Monoferric Transferrins,Serotransferrin,Transferrin B,Transferrin C,beta 2-Transferrin,beta-1 Metal-Binding Globulin,tau-Transferrin,Globulin, beta-1 Metal-Binding,Metal-Binding Globulin, beta-1,Transferrins, Monoferric,beta 1 Metal Binding Globulin,beta 2 Transferrin,tau Transferrin