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The nucleotide sequence of a cloned DNA coding for the 35-kDa polypeptide of the eye lens of the frog (Rana temporaria) has been determined. The sequence without connectors and poly(A) tract is 889 nucleotides in length and shows no homology with sequences coding for other classes of crystallins: alpha-, beta-, gamma- or delta-crystallins. The sequence contains one reading frame 675 nucleotides in length, an apparently intact 3'-non-translated region with the polyadenylation signal sequence and a poly(A) tract; the 5'-non-translated region is lost along with part of the coding region; this accounts for about 1/4 of the total mRNA length. The secondary structure prediction according to the Ptitsin - Finkelstein method shows the presence of predominantly beta-strands with only a few alpha-helical regions. We conclude that the 35-kDa polypeptide from the frog eye lens belongs to a new class of eye lens crystallins for which we propose the name epsilon-crystallin.
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
January 1984,
Doklady Akademii nauk SSSR,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
April 1999,
Biochimica et biophysica acta,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
October 1983,
FEBS letters,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
September 2002,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
January 1990,
Ontogenez,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
January 1983,
Doklady Akademii nauk SSSR,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
January 2002,
Ontogenez,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
January 2016,
Biochimica et biophysica acta,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
February 1985,
European journal of biochemistry,
S I Tomarev, and
R D Zinovieva, and
S M Dolgilevich, and
S V Luchin, and
A S Krayev, and
K G Skryabin, and
G G Gause
July 1978,
Experimental eye research,
