-transparent.png){kind=logo}
Murine monoclonal antibodies (MAB) against the idiotype (Id) of B lymphocyte malignancies are powerful reagents for the study of these diseases, and are potentially useful for treatment. Different strategies for the production of these anti-Id MAB have been compared. Initially, the Id Ig from nonsecreting B cell tumors was "rescued" by human X mouse or human X human hybridization. These somatic cell hybridizations resulted in the secretion of human Ig in 10 and 100% of the fusions, respectively. In a second step, anti-Id MAB were produced by using the "rescued" Id Ig as immunogen. A more streamlined approach is based on a one-step procedure in which the tumor cell suspension is used as immunogen. This method of immunization, coupled with a four-layer ELISA, results in the detection of anti-Id MAB in a frequency of approximately 1% of the total hybrids. By using a pool of 10 different anti-Id MAB, each reactive with the tumor of one patient, we searched for idiotypic relatedness among a panel of 50 additional tumors. No cross-reactions were found, indicating that our current strategy results in the identification of unique idiotypic determinants among human B cell tumors. Idiotypic Ig can be found in the serum of patients with B cell tumors. Among groups of patients, there is a wide spectrum of serum Id levels, ranging from less than 0.01 microgram/ml to greater than 500 micrograms/ml.
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
July 1985,
Journal of immunology (Baltimore, Md. : 1950),
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
January 1992,
Blood,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
January 2002,
Lupus,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
August 2001,
Wei sheng wu xue bao = Acta microbiologica Sinica,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
December 1984,
European journal of immunology,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
January 2011,
Immunology letters,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
January 1991,
Molecular immunology,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
December 1989,
Molecular immunology,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
November 1984,
Journal of immunological methods,
K Thielemans, and
D G Maloney, and
T Meeker, and
J Fujimoto, and
C Doss, and
R A Warnke, and
J Bindl, and
J Gralow, and
R A Miller, and
R Levy
November 1999,
Journal of agricultural and food chemistry,
