The plasmatic profiles of androgens, estradiol, and progesterone, together with gonads and SSC modifications, have been followed, throughout the post-reproductive period in two Rana esculenta populations, inhabiting, respectively, a mountain pond (Colfiorito) and a sea level lagoon (Lesina). Testosterone and progesterone progressively decrease in the blood until July, while estradiol shows, in both sexes, an increase in the same month. Testosterone depletion accounts for thumb pad atrophy in the male and probably, in both sexes, for the summer interruption of sexual behavior. The estradiol increase could be linked to the induction of vitellogenin synthesis by the liver or, alternatively, could act through negative feed-back on the brain centers involved in GnRF synthesis and therefore it could be responsible for an inhibition of LH release and, in turn, of androgen synthesis/secretion by the gonads. The last function can be framed in the endocrine regulation of the so-called "refractory period" which interrupts the breeding during the summer. The hematic level of progesterone is higher during the ovulation period and this is consistent with the role assigned to the hormone, i.e., the induction of jelly release from oviductal glands. The hormonal trends in the blood of the two frog populations are very similar, although some differences exist in the levels of testosterone and progesterone.