BIOMAT Database Logo BIOMAT Database Logo

[DNA replication in mammalian cells acted on by chemical, physical and biological factors. I. DNA damage and replication in LL line cells treated with formaldehyde]. 1983

B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ

The inhibition of DNA synthesis and the appearance of single-strand breaks and/or alkali-labile sites in DNA and DNA-membrane cross-links were observed after formaldehyde treatment of cultured LL-line cells. It was shown that supercoiling of cell chromatin is not affected under these conditions. The initiation of DNA replication after the exposure with 10(-4) M formaldehyde occurs also without disturbance. Under the higher concentration of formaldehyde (10(-3) M), DNA elongation was inhibited. It is suggested that cross-linking of DNA with other molecules and structures for example membranes, stabilizes DNA supercoiling (chromatine). This conformational stability is essential for normal initiation of DNA replication, although the parenteral DNA contains many lesions in its primary and secondary structures.

UI MeSH Term Description Entries
D007739 L Cells A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS. Earle's Strain L Cells,L Cell Line,L Cells (Cell Line),L-Cell Line,L-Cells,L-Cells, Cell Line,L929 Cell Line,L929 Cells,NCTC Clone 929 Cells,NCTC Clone 929 of Strain L Cells,Strain L Cells,Cell Line L-Cell,Cell Line L-Cells,Cell Line, L,Cell Line, L929,Cell Lines, L,Cell, L,Cell, L (Cell Line),Cell, L929,Cell, Strain L,Cells, L,Cells, L (Cell Line),Cells, L929,Cells, Strain L,L Cell,L Cell (Cell Line),L Cell Lines,L Cell, Strain,L Cells, Cell Line,L Cells, Strain,L-Cell,L-Cell Lines,L-Cell, Cell Line,L929 Cell,Strain L Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002843 Chromatin The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. Chromatins
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005557 Formaldehyde A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717) Formalin,Formol,Methanal,Oxomethane
D005720 Gamma Rays Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source. Gamma Wave,Gamma Radiation,Nuclear X-Rays,Radiation, Gamma,X-Rays, Nuclear,Gamma Radiations,Gamma Ray,Gamma Waves,Nuclear X Rays,Nuclear X-Ray,Ray, Gamma,Wave, Gamma,Waves, Gamma,X Rays, Nuclear,X-Ray, Nuclear
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells exposed to physical, chemical or biological factors. II. DNA-complex and nucleoid recovery and DNA replication after gamma irradiation].
November 1984, Tsitologiia,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
Perspectives on formaldehyde dysregulation: Mitochondrial DNA damage and repair in mammalian cells.
September 2021, DNA repair,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells after the action of physical, chemical or biological factors. VI. The recovery of DNA synthesis in a culture of irradiated cells].
January 1991, Tsitologiia,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells after exposure to factors of a physical, chemical or biological nature. III. 5-Fluorodeoxyuridine induces DNA synthesis in cells not suppressed by gamma irradiation].
February 1985, Tsitologiia,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells exposed to the action of physical, chemical or biological factors. IV. 5-fluorodeoxyuridine modifies the effect of hyperthermia on DNA synthesis and the survival of HeLa cells].
August 1985, Tsitologiia,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
Relationship between DNA damage and survival in formaldehyde-treated mouse cells.
November 1980, Mutation research,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
Chemical nature of in vivo DNA base damage in hydrogen peroxide-treated mammalian cells.
March 1991, Archives of biochemistry and biophysics,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells after the action of factors of a physical, chemical or biological nature. V. The structural reorganization of replicating DNA in HeLa cells after incubation with 5-fluorodeoxyuridine].
September 1986, Tsitologiia,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
[DNA replication in mammalian cells].
July 1989, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
B I Synzynys, and M Pirshel, and M Sedliaková, and S F Trofimova, and A M Poverennyĭ
DNA synthesis, mutagenesis, DNA damage, and cytotoxicity in cultured mammalian cells treated with alkylating agents.
March 1980, Cancer research,
Copied contents to your clipboard!