BIOMAT Database Logo BIOMAT Database Logo

Imprint immunofixation of IgG subclasses, using monoclonal subclass-specific antibodies. 1983

F Vartdal, and P Bird

Monoclonal mouse hybridoma antibodies to human IgG subclasses 1-4 were used in an imprint immunofixation (IIF) technique to characterize the IgG subclass of a panel of IgG myeloma and normal sera separated by thin-layer electrofocusing. The results show that the antisera were IgG subclass-specific by this technique and that IIF can be a useful method in identifying IgG subclasses of IgG myeloma proteins in whole sera.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D009194 Myeloma Proteins Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA. M Components,Proteins, Myeloma
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D001345 Autoradiography The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed) Radioautography

Related Publications

F Vartdal, and P Bird
Porcine IgG. Isolation of two IgG-subclasses and anti-IgG class- and subclass-specific antibodies.
June 1986, Molecular immunology,
F Vartdal, and P Bird
Use of monoclonal antibodies to quantify subclasses of human IgG. II. Enzyme immunoassay to define antigen specific (anti-filarial) IgG subclass antibodies.
November 1986, Journal of immunological methods,
F Vartdal, and P Bird
IgG subclass typing by immunofixation.
May 1997, Clinica chimica acta; international journal of clinical chemistry,
F Vartdal, and P Bird
Human IgG subclass-specific epitopes recognised by murine monoclonal antibodies.
January 1986, Monographs in allergy,
F Vartdal, and P Bird
Preparation of monoclonal antibodies specific for the subclasses of canine IgG.
September 1994, Research in veterinary science,
F Vartdal, and P Bird
Antigenic variants of human IgG subclasses. Restriction of murine monoclonal IgG subclass antisera.
February 1987, Scandinavian journal of immunology,
F Vartdal, and P Bird
Restrictive murine monoclonal IgG subclass antisera revealing antigenic variants of human IgG subclasses.
January 1988, Monographs in allergy,
F Vartdal, and P Bird
Subclass typing of IgG paraproteins by immunofixation electrophoresis.
October 1995, Clinical chemistry,
F Vartdal, and P Bird
Human Allergen-Specific IgG Subclass Antibodies Measured Using ImmunoCAP Technology.
January 2017, International archives of allergy and immunology,
F Vartdal, and P Bird
ELISA measurement of IgG subclass production in culture supernatants using monoclonal antibodies.
November 1987, Journal of immunological methods,
Copied contents to your clipboard!