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We have investigated the spontaneous release of heme from chromatographically homogeneous horseradish peroxidase A2 at varying temperature, pH and iron ligands. A "biphasic" rate of heme release was observed under all conditions. Upon further purification of HRP A2, a component was isolated that was homogeneous by polyacrylamide gel electrophoresis and exhibited a single first order rate of heme release. The rate of the release increased with pH and temperature, decreased in the presence of cyanide and increased slightly in the presence of fluoride. These results are consistent with the idea that the rate of heme release is a measure of the flexibility of the protein lining the heme "pocket" with iron bonding playing a secondary, though important role in heme-protein interactions.
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
April 2000,
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
April 1997,
Biochemistry. Biokhimiia,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
April 1976,
Biochimica et biophysica acta,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
October 1997,
Doklady Akademii nauk,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
May 1972,
Biochimica et biophysica acta,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
May 2005,
Biochimica et biophysica acta,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
December 1980,
Biochemical and biophysical research communications,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
November 1986,
FEBS letters,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
August 1983,
Photochemistry and photobiology,
M L Smith, and
K Hjortsberg, and
P I Ohlsson, and
K G Paul
July 1991,
Biochemical and biophysical research communications,
