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Methanobacterium thermoautotrophicum was grown in phosphate-limited chemostat cultures at a dilution rate corresponding to a doubling time of 13.2 h. The cyclic-2,3-diphospho-D-glycerate content of these cells was 8 to 10-fold lower than that of cells grown in batch cultures having a doubling time of 11.5 h. This metabolite accounted for 5% of cell dry weight during batch growth on 2 mM phosphate. In the chemostat the steady-state concentration of phosphate was 4 microM, showing that this methanogen is adapted to highly efficient growth at low phosphate concentrations. Since growth rates were similar in both cultures, the growth rate clearly does not depend on intracellular levels of cyclic-2,3-diphosphoglycerate.
R J Seely, and
R D Krueger, and
D E Fahrney
October 1984,
Journal of bacteriology,
R J Seely, and
R D Krueger, and
D E Fahrney
March 1992,
Biochemistry,
R J Seely, and
R D Krueger, and
D E Fahrney
September 1986,
The Journal of biological chemistry,
R J Seely, and
R D Krueger, and
D E Fahrney
July 1993,
Journal of bacteriology,
R J Seely, and
R D Krueger, and
D E Fahrney
July 1986,
Journal of bacteriology,
R J Seely, and
R D Krueger, and
D E Fahrney
March 1988,
Polskie Archiwum Medycyny Wewnetrznej,
R J Seely, and
R D Krueger, and
D E Fahrney
September 1983,
Proceedings of the National Academy of Sciences of the United States of America,
R J Seely, and
R D Krueger, and
D E Fahrney
January 1984,
International surgery,
R J Seely, and
R D Krueger, and
D E Fahrney
January 1975,
Verhandlungen der Deutschen Gesellschaft fur Innere Medizin,
R J Seely, and
R D Krueger, and
D E Fahrney
May 1977,
Diabetes,
