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Several rat liver cytochromes P-450 have been substantially purified in a one-step immunoadsorption procedure using Sepharose-bound monoclonal antibodies (MAbs) to the major forms of rat liver cytochrome P-450 induced by 3-methylcholanthrene and phenobarbital (MC-P-450 and PB-P-450, respectively). When mixed with solubilized rat liver microsomes the immunoadsorbent based on the MAb to MC-P-450 binds two polypeptides of MW 56,000 and 57,000 while the immunoadsorbent made with the MAb to PB-P-450 absorbs a species of MW 54,000. These polypeptides are readily desorbed by 0.1 M glycine (pH 3.0). Isolation of MAb-specific cytochrome P-450 isozymes by this method has applications in numerous phases of cytochrome P-450 research.
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
February 1984,
The Journal of biological chemistry,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
September 1984,
Biochemical and biophysical research communications,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
January 1986,
Advances in experimental medicine and biology,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
May 1986,
Biochemistry,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
January 1988,
Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
April 1985,
Science (New York, N.Y.),
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
January 1988,
Biochemical pharmacology,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
February 1988,
Xenobiotica; the fate of foreign compounds in biological systems,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
December 1988,
Biochemical pharmacology,
F K Friedman, and
R C Robinson, and
S S Park, and
H V Gelboin
June 1996,
Molecular microbiology,
