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An affinity chromatographic method for the determination of glycosylated hemoglobin (HbA1) was evaluated. The procedure was shown to be precise, the within- and between-assay coefficients of variation being less than 5%. It was also shown to correlate well with electrophoresis (r = 0.968) and ion-exchange chromatography (r = 0.916). An inverse relationship was shown to exist between increasing temperature and HbA1 levels measured by affinity chromatography. A statistically significant difference was found for samples run at 20 degrees C and 25 degrees C respectively, suggesting that the method should be run in a temperature-controlled environment. The affinity procedure was also shown not to be affected by the type of anticoagulant, the concentration of hemoglobin in the hemolysate, and acetylation.
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
December 1982,
Clinical biochemistry,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
October 2000,
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R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
January 1987,
Vutreshni bolesti,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
January 1983,
Hemoglobin,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
September 1983,
Lijecnicki vjesnik,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
January 1984,
Journal of perinatal medicine,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
December 1980,
The New England journal of medicine,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
January 1982,
Diabetes care,
R W Yatscoff, and
G J Tevaarwerk, and
C L Clarson, and
L M Warnock
February 1983,
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R W Yatscoff, and
G J Tevaarwerk, and
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January 1995,
Medecine tropicale : revue du Corps de sante colonial,
