Biomaterial Database

Physical studies of lanthanide binding to concanavalin A. 1978

C E Richardson, and W D Behnke

The binding of lanthanide ions to concanavalin A has been studied using circular dichroic, magnetic circular dichroic and fluorescence spectroscopies. A Scatchard analysis of the fluorescence enhancement of Tb3+ binding reveal the presence of two classes of sites. One strong binding site (S3) is present per protomer which is not affected by addition of transition metals or calcium. There are at least three additional weak binding sites per protomer, one of which may be S1. There is virtually no energy transfer from Tb3+ to Co2+ in cobalt-substituted concanavalin A. The S3 site, therefore, must be at least 39 A distant from S1. The circular dichroic spectrum of Co2+ concanavalin A is altered by addition of Gd3+ in a manner consistent with competition for S1. The magnetic circular dichroic spectrum of Pr3+ bound to concanavalin A is not affected by excess Ca2+ or the addition of specific carbohydrate effectors. There is no evidence for lanthanide ion binding to S2.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008027	Light	That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.	Light, Visible,Photoradiation,Radiation, Visible,Visible Radiation,Photoradiations,Radiations, Visible,Visible Light,Visible Radiations
D008674	Metals, Rare Earth	A group of elements that include SCANDIUM; YTTRIUM; and the LANTHANOID SERIES ELEMENTS. Historically, the rare earth metals got their name from the fact that they were never found in their pure elemental form, but as an oxide. In addition, they were very difficult to purify. They are not truly rare and comprise about 25% of the metals in the earth's crust.	Rare Earth Metal,Rare Earth Metals,Earth Metal, Rare,Earth Metals, Rare,Metal, Rare Earth
D011221	Praseodymium	An element of the rare earth family of metals. It has the atomic symbol Pr, atomic number 59, and atomic weight 140.91.
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D005682	Gadolinium	An element of the rare earth family of metals. It has the atomic symbol Gd, atomic number 64, and atomic weight 157.25. Its oxide is used in the control rods of some nuclear reactors.
D012542	Scattering, Radiation	The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Radiation Scattering,Radiation Scatterings,Scatterings, Radiation
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013725	Terbium	An element of the rare earth family of metals. It has the atomic symbol Tb, atomic number 65, and atomic weight 158.92.