Biomaterial Database

Quantitation of human serum antithyroglobulin antibodies by a two-site radioimmunoassay. 1978

J P Leonard, and F Taymans, and C Beckers

A two-site solid-phase radioimmunoassay for measuring serum antithyroglobulin antibodies applicable to human serum is described. The sequential steps of the procedure include the adsorption of thyroglobulin (Tg) on polystyrene tubes and the binding of the antithyroglobulin antibody to solid phase followed by the fixation of labelled thyroglobulin. The optimal experimental conditions for the adsorption of unlabelled Tg, the uptake of the antibodies and the fixation of labelled Tg have been defined. The test is specific for antithyroglobulin antibody and its reproducibility and precision are satisfactory. The radioassay showed a good correlation with the hemagglutination method and, also, was found to be more sensitive. Levels of antithyroglobulin IgG in positive sera ranged from 0.3 microgram/ml to several mg/ml. In contrast to the double antibody radioimmunoassay for antithyroglobulin antibodies, this solid-phase radioassay is easier to perform; moreover, it is relatively independent of the quality, the purity and the specific activity of the tracer.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D013954	Thyroglobulin
D013959	Thyroid Diseases	Pathological processes involving the THYROID GLAND.	Disease, Thyroid,Diseases, Thyroid,Thyroid Disease