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360-MHz proton nuclear magnetic resonance spectra were recorded of 10 sialyl-oligosaccharides isolated from urine of sialidosis patients. Their structures are related to the complex asparagine-linked glycan chains of glycoproteins. By correlation of these spectra and comparison with spectra of reference glycopeptides and sialyl-lactose isomers it was possible to assign all signals belonging to anomeric, mannose H-2, sialic acid H-3 and N-acetyl protons. The number of the consituting monosaccharide residues of the oligomers can be obtained by integration of the above-mentioned signals. The chemical shifts of the anomeric and mannose H-2 protons give information about the type of glycan structure (mono-, bi-, triantennary) and the presence of terminal sialic acid at each of the antennas. The chemical shifts of sialic acid H-3 protons are typical for sialic acid residues in 2 leads to 3 or 2 leads to 6 linkage to galactose.
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
January 1980,
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L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
May 1977,
European journal of biochemistry,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
March 1980,
Journal of molecular biology,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
October 1979,
Journal of inorganic biochemistry,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
June 1978,
Minerva pediatrica,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
March 1979,
Archives francaises de pediatrie,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
February 1983,
Journal of molecular biology,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
June 1980,
The Biochemical journal,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
November 1981,
Biochemistry,
L Dorland, and
J Haverkamp, and
J F Viliegenthart, and
G Strecker, and
J C Michalski, and
B Fournet, and
G Spik, and
J Montreuil
April 1984,
European journal of biochemistry,
