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Amiloride, a K+-sparing diuretic, and diltiazem, a Ca2+ channel antagonist, have both been reported to inhibit Na+ transport-associated processes in different subcellular membrane systems. In this report, similar inhibitory effects of both agents are demonstrated on Na+-induced Ca2+ release from rabbit heart mitochondria and on Na+ uptake in a kidney medulla microsomal preparation. Both amiloride and diltiazem produce 50% inhibition of Na+ uptake in kidney microsomes at the same concentrations. Heart mitochondrial Na+-induced Ca2+ release was 50% inhibited by 6 microM diltiazem and 200 microM amiloride. No effects of either agent on mitochondrial respiratory activity were observed. The results suggest a specific effect of both drugs on a Na+-binding site associated with an antiport exchange process. These data also extend previous observations suggesting the use of these agents as tools to define further ion transport mechanisms in biological membranes.
L A Sordahl, and
E F LaBelle, and
K A Rex
May 1985,
Molecular pharmacology,
L A Sordahl, and
E F LaBelle, and
K A Rex
October 1985,
Cell calcium,
L A Sordahl, and
E F LaBelle, and
K A Rex
January 1989,
The American journal of physiology,
L A Sordahl, and
E F LaBelle, and
K A Rex
September 1983,
Molecular pharmacology,
L A Sordahl, and
E F LaBelle, and
K A Rex
October 1984,
European journal of pharmacology,
L A Sordahl, and
E F LaBelle, and
K A Rex
June 1990,
Experimental cell research,
L A Sordahl, and
E F LaBelle, and
K A Rex
October 1983,
FEBS letters,
L A Sordahl, and
E F LaBelle, and
K A Rex
April 1987,
Life sciences,
L A Sordahl, and
E F LaBelle, and
K A Rex
June 1990,
Cardiologia (Rome, Italy),
L A Sordahl, and
E F LaBelle, and
K A Rex
February 1988,
The American journal of physiology,
