Actinomadura cultures were isolated from soil samples collected in Turkmenistan, the Moscow, Kursk and Volgograd regions. The cultures were described as representatives of a new species (Actinomadura recticatena, Preobrazhenskaya et Galatenko, sp. nov). The hydrolysates of intact cells of the cultures contain mesodiaminopumelic acid, galactose and madurose. The species is characterized by straight spore chains, wrinkled spore surface, cream-colored aerial mycelium and brown substrate mycelium with pinkish or slightly violet shade. The cultures produce no soluble pigments, including the melanoid ones. The red-violet fraction of the pigments responsible for the pinkish and violet shades of the substrate mycelium belongs to the antibiotics of the griseorodin-rubromycin group.