Biomaterial Database

RNA synthesis in rat liver cells with different DNA contents. 1978

J M Collins

The DNA content of mature rat liver parenchymal cells falls into three distinct classes with 1:2:4 amounts of DNA. These classes represent 2 N, 4 N, and 8 N cells. We have measured the changing proportions of these cells from 0.75 to 24 months of age. We have obtained a useful approximation for the numbers of each ploidy class, for cellular preparations from liver, as a function of the amount of DNA and age. Multiple linear regression analysis revealed that the average DNA content per cell is given by the relationship DNA/cell = 41.28(1/2)--324.14 Age1/4 + 499.37 Age1/8--207.24, with a multiple correlation coefficient of 0.990. Measurements of the amount of RNA synthesized in a 15-min period revealed that the relative proportions were 1.2:1.0 for 4 N:2 N cells of young rats, 1.3:1.1.:1.0 for 8 N:4 N:2 N cells of intermediate ages, and 1.1:1.0 for 8 N:4 N cells of old rats. The absolute amounts of RNA synthesized by each ploidy class declined with age.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D009929	Organ Size	The measurement of an organ in volume, mass, or heaviness.	Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D011003	Ploidies	The degree of replication of the chromosome set in the karyotype.	Ploidy
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012313	RNA	A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)	RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In