BIOMAT Database Logo BIOMAT Database Logo

Reverse phase high pressure liquid chromatography for the separation of peptide hormone diastereoisomers. 1978

B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby

Diastereoisomers of specifically labeled oxytocin derivatives were resolved using reverse phase high pressure liquid chromatography. The peptides [1-hemi-DL-[alpha-2H]cystine]oxytocin, [6-hemi-DL-[alpha-2H]cystine]oxytocin, [2-DL-[alpha-2H]tyrosine]oxytocin and[8-DL-[2-13C]leucine]oxytocin were readily separated using the conditions described. The diastereoisomers of the oxytocin analog [3-DL-[2-13C]leucine]oxytocin also demonstrated baseline resolution under the same conditions. The procedure offers the investigator a rapid method for screening synthetic oxytocin peptides for undesirable diastereoisomeric by-products.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D010121 Oxytocin A nonapeptide hormone released from the neurohypophysis (PITUITARY GLAND, POSTERIOR). It differs from VASOPRESSIN by two amino acids at residues 3 and 8. Oxytocin acts on SMOOTH MUSCLE CELLS, such as causing UTERINE CONTRACTIONS and MILK EJECTION. Ocytocin,Pitocin,Syntocinon
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D013237 Stereoisomerism The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Molecular Stereochemistry,Stereoisomers,Stereochemistry, Molecular,Stereoisomer

Related Publications

B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
The separation of peptide hormone diastereoisomers by reverse phase high pressure liquid chromatography. Factors affecting separation of oxytocin and its diastereoisomers--structural implications.
January 1979, International journal of peptide and protein research,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Peptide mapping by reverse-phase high pressure liquid chromatography.
January 1984, Methods in molecular biology (Clifton, N.J.),
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Separation of phycobiliprotein subunits by reverse-phase high-pressure liquid chromatography.
August 1990, Analytical biochemistry,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography.
July 1983, Journal of clinical microbiology,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Rapid separation of acetylated oligosaccharides by reverse-phase high-pressure liquid chromatography.
August 1979, Analytical biochemistry,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
The separation of glutathione transferase subunits by using reverse-phase high-pressure liquid chromatography.
July 1987, The Biochemical journal,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Separation of penicillin and cephalosporin diastereoisomers by reversed-phase high-performance liquid chromatography.
November 1978, Journal of chromatography,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography.
October 1982, Steroids,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Peptide separation by reversed-phase high-performance liquid chromatography.
January 1983, Molecular and cellular biochemistry,
B Larsen, and V Viswanatha, and S Y Chang, and V J Hruby
Automated isocratic separation of phenylthiohydantoin-amino acids by tandem reverse phase high-pressure liquid chromatography columns.
September 1980, Analytical biochemistry,
Copied contents to your clipboard!