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The mechanism by which chloride stimulates adenylate cyclase was investigated. Depletion of GDP increased basal adenylate cyclase activity and reduced the stimulation by isoprenaline. Restoration of bound GDP partially reversed these effects. Chloride stimulated cyclase activity by the same proportion in control, GDP-depleted and GDP-restored preparations, as did Gpp(NH)p. Fluoride increased adenylate cyclase activity to the same final level in both GDP-depleted and GDP-restored membranes; addition of Gpp(NH)p as well as fluoride had no further effect. Solubilisation of adenylate cyclase reduced the stimulatory effect of Gpp(NH)p only slightly, but greatly attenuated the activation by chloride. We conclude that chloride does not stimulate cyclase activity by an action on GDP exchange. Activation by chloride may be due to a disrupting or chaotropic effect on membrane/protein interactions.
S E Harding, and
L Leclerc, and
P Harris
November 1987,
Biulleten' eksperimental'noi biologii i meditsiny,
S E Harding, and
L Leclerc, and
P Harris
August 1981,
Biokhimiia (Moscow, Russia),
S E Harding, and
L Leclerc, and
P Harris
April 1974,
The Journal of clinical investigation,
S E Harding, and
L Leclerc, and
P Harris
September 1977,
Molecular pharmacology,
S E Harding, and
L Leclerc, and
P Harris
August 1984,
FEBS letters,
S E Harding, and
L Leclerc, and
P Harris
March 1976,
Brain research,
S E Harding, and
L Leclerc, and
P Harris
April 1987,
Pharmacology & toxicology,
S E Harding, and
L Leclerc, and
P Harris
January 1982,
Folia morphologica,
S E Harding, and
L Leclerc, and
P Harris
January 1980,
Agressologie: revue internationale de physio-biologie et de pharmacologie appliquees aux effets de l'agression,
S E Harding, and
L Leclerc, and
P Harris
May 1975,
Proceedings of the National Academy of Sciences of the United States of America,
