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By using a kinetic assay, we have examined the role of antibody in the lysis of rabbit erythrocytes (RaRBC) through the alternative complement (C) pathway. Sera from some hypogammaglobulinemic (Hgamma) humans and all agammaglobulinemic chickens tested had subnormal activity in the assay. Heated normal human or chicken sera, but not heated Hgamma sera, restored activity to deficient Hgamma serum and initiated hemolysis in the presence of rabbit serum as C source. Absorption of heated normal human serum with RaRBC, but not with sheep erythrocytes or zymosan, removed its ability to reconstitute deficient Hgamma serum. Normal hemolytic activity could be resotred to Hgamma serum with human IgM, IgG, or colostral IgA, with goat anti-RaRBC IgG, or with an eluate from serum-sensitized RaRBC, but not with myeloma IgA. Restoration of hemolytic activity to Hgamma serum could be achieved in a dose-dependent fashion with the F(ab')2 fragment of IgG. These results suggest that antibody exerts a significant rate-limiting effect on alternative pathway activity in the RaRBC lytic system. This raises the possibility that antibody may be required for efficient alternative pathway activity in vivo and that the pyogenic infections that occur in Hgamma individuals are due to inefficient activation and fixation of C3 through either the classical or alternative pathway.
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
July 1978,
Journal of immunology (Baltimore, Md. : 1950),
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
October 1979,
Archives internationales de physiologie et de biochimie,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
February 1978,
American journal of clinical pathology,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
January 2021,
Methods in molecular biology (Clifton, N.J.),
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
September 1993,
Veterinary immunology and immunopathology,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
May 1985,
Journal of immunology (Baltimore, Md. : 1950),
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
April 1989,
The Journal of investigative dermatology,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
January 1983,
Springer seminars in immunopathology,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
January 1985,
Developmental and comparative immunology,
R B Polhill, and
S L Newman, and
K M Pruitt, and
R B Johnston
January 1989,
Dermatologica,
