Evaluation of the enzymatic ammonia method for urine on the Du Pont automatic clinical analyzer. 1978

F R Hindriks, and A Groen

The enzymatic method for ammonia with the Du Pont Automatic Clinical Analyzer was tested in our laboratory. The method is very suitable for urine samples although the method was released by Du Pont only for plasma samples. The results correlated well with those obtained by a method based on the Berthelot indophenol reaction and by a method based on the use of an ammonium electrode, which has an ammonium ion selective membrane. The day-to-day coefficient of variation is about 2 and 4% at concentration levels of 100 and 50 mumol/1 respectively. The measured and relative ammonia concentration are linearly related. Recovery of added known amounts of ammonium is complete and no interference from glucose, urea, creatinine and uric acid could be demonstrated. Recommendations are given for handling blood and urine samples before analysis.

UI MeSH Term Description Entries
D004566 Electrodes Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum. Anode,Anode Materials,Cathode,Cathode Materials,Anode Material,Anodes,Cathode Material,Cathodes,Electrode,Material, Anode,Material, Cathode
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000641 Ammonia A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
D001322 Autoanalysis Method of analyzing chemicals using automation. Autoanalyses

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