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A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites.
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
November 1985,
The Journal of biological chemistry,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
September 1989,
Journal of steroid biochemistry,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
June 1987,
FEBS letters,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
October 1987,
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
May 1984,
Biochemical and biophysical research communications,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
March 1980,
Proceedings of the National Academy of Sciences of the United States of America,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
July 1980,
The American journal of physiology,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
November 1986,
European journal of biochemistry,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
July 1983,
Biochemistry,
R U Simpson, and
J K Wichmann, and
H E Paaren, and
H K Schnoes, and
H F DeLuca
April 1977,
Biochemical and biophysical research communications,
