Determination of cobalt in biological materials by voltammetry and electrothermal atomic absorption spectrometry. 1984

R Heinrich, and J Angerer

For industrial purposes cobalt is used to a large extent. About 2,300 persons are occupationally exposed to this carcinogenic metal in Western Germany. As reliable analytic methods for biological monitoring are not available we developed procedures for analyzing cobalt in whole blood and urine by using two independent methods, voltammetry and ETAAS. For ETAAS-analysis of urine the cobalt content is chelatized and extracted in an organic solvent. This clean-up step enables us to calibrate with aqueous standards.--Samples of whole blood are directly injected into the graphite tube after being diluted with a homogeniziser. For their gentle thermal decomposition we use a temperature/time programme containing six steps. Both kinds of sample treatments are uncomplicated and permit routine applications. For voltammetric determination of cobalt in urine and blood the biological material must be completely mineralized. The dry residue is dissolved in a NH4Cl/NH3 solution. Cobalt is chelatized with 2,3-butanedione-dioxime and preconcentrated by adsorption at the hanging mercury electrode. By scanning the potential into negative direction the cobalt complex is reduced. The resulting signal can be used for the quantitative determination. For comparison of both methods we have analyzed blood and urine samples of occupationally exposed persons. We found very good correlations with a statistical significance at the level of 0.01%.

UI MeSH Term Description Entries
D003035 Cobalt A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis. Cobalt-59,Cobalt 59
D004563 Electrochemistry The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes. Electrochemistries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013054 Spectrophotometry, Atomic Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed. Spectrophotometry, Atomic Absorption,AA Spectrophotometry,AE Spectrophotometry,Atomic Absorption Spectrophotometry,Atomic Emission Spectrophotometry,Atomic Spectrophotometry,Inductively Coupled Plasma Atomic Emission Spectrophotometry,Inductively Coupled Plasma Atomic Emission Spectroscopy,Spectrophotometry, Atomic Emission,AA Spectrophotometries,AE Spectrophotometries,Absorption Spectrophotometry, Atomic,Emission Spectrophotometry, Atomic,Spectrophotometries, AA,Spectrophotometries, AE,Spectrophotometry, AA,Spectrophotometry, AE

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