-transparent.png){kind=logo}
A sequential assignment procedure is outlined, based on two-dimensional NOE ( NOESY ) and two-dimensional J-correlated spectroscopy ( COSY ), for assigning the nonexchangeable proton resonances in NMR spectra of oligonucleotides. As presented here the method is generally applicable to right-handed helical oligonucleotides of intermediate size. We applied it to a lac operator DNA fragment consisting of d( TGAGCGG ) and d( CCGCTCA ) and obtained complete assignments for the adenine H8, guanine H8, cytosine H6 and H5, thymine H6 and 5-methyl, and the deoxyribose H1', H2', H2", H3', and H4' resonances, as well as some H5', H5" (pairwise) assignments. These assignments are required for the analysis of two-dimensional NOE and J-coupling data in terms of the solution structure of oligonucleotides.
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
January 1983,
Biopolymers,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
November 1986,
Biochemistry,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
November 1986,
FEBS letters,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
March 1991,
Biochemical and biophysical research communications,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
December 1991,
Biochemistry,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
August 1985,
Biochemistry,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
March 1982,
Journal of molecular biology,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
January 1994,
European journal of biochemistry,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
February 1990,
European journal of biochemistry,
R M Scheek, and
R Boelens, and
N Russo, and
J H van Boom, and
R Kaptein
December 1991,
European journal of biochemistry,
