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HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first phase which is relatively brief (less than 5 min) is reflected in a diffuse, irregular organization of nucleosomes on new DNA immediately postreplicatively . The second phase which lasts from 5 to 30 min postreplication is characterized by a normal repeat length for the nucleosomes which are nonetheless more weakly bound than bulk nucleosomes. This is reflected in increased sliding during nuclease digestion as well as increased nuclease sensitivity and the presence of easily dissociated histones which has been described by other workers.
P A Smith, and
V Jackson, and
R Chalkley
October 1983,
The Journal of biological chemistry,
P A Smith, and
V Jackson, and
R Chalkley
November 1978,
Cell,
P A Smith, and
V Jackson, and
R Chalkley
February 1980,
Journal of molecular biology,
P A Smith, and
V Jackson, and
R Chalkley
January 1974,
Cold Spring Harbor symposia on quantitative biology,
P A Smith, and
V Jackson, and
R Chalkley
June 1980,
Proceedings of the National Academy of Sciences of the United States of America,
P A Smith, and
V Jackson, and
R Chalkley
February 1995,
Experimental cell research,
P A Smith, and
V Jackson, and
R Chalkley
August 1984,
Nucleic acids research,
P A Smith, and
V Jackson, and
R Chalkley
December 1978,
Proceedings of the National Academy of Sciences of the United States of America,
P A Smith, and
V Jackson, and
R Chalkley
January 2020,
Acta of bioengineering and biomechanics,
P A Smith, and
V Jackson, and
R Chalkley
January 1989,
Biochimica et biophysica acta,
