Biomaterial Database

Intracellular injection with horseradish peroxidase of physiologically characterized stellate and bushy cells in slices of mouse anteroventral cochlear nucleus. 1984

S H Wu, and D Oertel

Nissl-stained tissue from brain slice preparations of the anteroventral cochlear nucleus of the mouse resembles tissue fixed in situ. Multipolar, spherical, globular, and granule cells can be distinguished after intracellular injection with horseradish peroxidase (HRP). Stellate cells have relatively large dendritic fields; their axons have collaterals which terminate within the cochlear nuclear complex. Bushy cells have smaller dendritic fields; where they can be seen, axons have no collaterals. Granule cells have few short dendrites; their very fine axons branch close to the cell body and could be followed only for short distances. Intracellular recordings from six stellate cells labeled by intracellular injection of HRP revealed that they have linear current-voltage relationships around the resting potential and that they respond to suprathreshold depolarization with large, regularly firing action potentials. Intracellular recordings from four bushy cells, also labeled by injection of HRP, showed that these cells have nonlinear current-voltage relationships around the resting potential and that they respond to suprathreshold depolarizations with only one or two small action potentials. The anatomical and physiological features of bushy cells reduce summing in time and space and make bushy cells well suited to preserve the firing patterns of auditory nerve inputs. The anatomical and physiological features of stellate cells, in contrast, allow summing in time and space.

UI	MeSH Term	Description	Entries
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D003056	Cochlear Nerve	The cochlear part of the 8th cranial nerve (VESTIBULOCOCHLEAR NERVE). The cochlear nerve fibers originate from neurons of the SPIRAL GANGLION and project peripherally to cochlear hair cells and centrally to the cochlear nuclei (COCHLEAR NUCLEUS) of the BRAIN STEM. They mediate the sense of hearing.	Acoustic Nerve,Auditory Nerve,Acoustic Nerves,Auditory Nerves,Cochlear Nerves,Nerve, Acoustic,Nerve, Auditory,Nerve, Cochlear,Nerves, Acoustic,Nerves, Auditory,Nerves, Cochlear
D003712	Dendrites	Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS.	Dendrite
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon
D013233	Stellate Ganglion	A paravertebral sympathetic ganglion formed by the fusion of the inferior cervical and first thoracic ganglia.	Cervicothoracic Ganglion,Cervicothoracic Ganglia,Stellate Ganglia,Ganglia, Cervicothoracic,Ganglia, Stellate,Ganglias, Stellate,Ganglion, Cervicothoracic,Ganglion, Stellate,Stellate Ganglias
D013569	Synapses	Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.	Synapse