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The sedimentation coefficient and buoyant density of mononucleosomes from the replicating chromatin of heated (45 degrees) and unheated HeLa cells were determined. It was observed that both physical parameters were the same (S = 10.8 and p = 1.165 g/cm3) for nucleosome particles from replicating and mature chromatin in heated and unbeated cells. The size of DNA in the nascent and mature nucleosome from heated or unheated cells was observed to be 145 base pairs of double-stranded DNA. A significant fraction of nucleosomal DNA from heated cells was observed at subnucleosomal sizes, principally at 125 base pairs of DNA. It is concluded that 45 degrees thermal shock does not alter appreciably the subunit structure of chromatin at the replication fork.
R L Warters, and
O L Stone
May 1975,
Analytical biochemistry,
R L Warters, and
O L Stone
January 1978,
Methods in cell biology,
R L Warters, and
O L Stone
December 1973,
FEBS letters,
R L Warters, and
O L Stone
January 1976,
Analytical biochemistry,
R L Warters, and
O L Stone
February 1972,
Journal of molecular biology,
R L Warters, and
O L Stone
March 1975,
Journal of molecular biology,
R L Warters, and
O L Stone
May 1973,
Acta virologica,
R L Warters, and
O L Stone
March 1977,
Molecular biology reports,
R L Warters, and
O L Stone
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
R L Warters, and
O L Stone
July 1974,
Biochimica et biophysica acta,
