-transparent.png){kind=logo}
Temperature-dependent spin changes of the heme iron atom on cytochrome P-450scc were studied by optical absorption and circular dichroism measurements. The optical absorption and circular dichroism spectra of cholesterol-free cytochrome P-450scc did not change between 10 and 26 degrees C. In contrast, the absorbance at 390 nm and the ellipticity at 330 nm of cholesterol-bound cytochrome P-450scc decreased upon temperature elevation, and the absorbance at 424 nm correspondingly increased. These spectral changes were reversible in respect of temperature. The far-ultraviolet circular dichroism spectra of both cholesterol-bound and -free cytochrome P-450scc were not affected by temperature. In addition, bound cholesterol molecule is not released from the cytochrome molecule by increasing temperature. From these results, we propose that temperature modulates specific interactions between the heme protein and bound cholesterol rather than the gross secondary structural changes of the protein.
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
June 1988,
FEBS letters,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
August 1987,
Biochimica et biophysica acta,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
January 1976,
Advances in experimental medicine and biology,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
March 1979,
Biochimica et biophysica acta,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
June 1991,
The Journal of steroid biochemistry and molecular biology,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
January 1991,
The International journal of biochemistry,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
February 1981,
Biochemistry,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
October 1980,
European journal of biochemistry,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
January 1981,
Steroids,
H Hasumi, and
F Yamakura, and
S Nakamura, and
K Suzuki, and
T Kimura
May 1971,
Biochemical and biophysical research communications,
