Biomaterial Database

Enkephalin heptapeptide (Tyr-Gly-Gly-Phe-Met-Arg-Phe)-induced bronchospasm in guinea pigs: a non-naloxone reversible phenomenon. 1984

R E Chipkin, and R W Chapman

Enkephalin heptapeptide (Tyr-Gly-Gly-Phe-Met-Arg-Phe; MEAP ), is an enkephalin-related peptide derived from pre-pro-enkephalin A. Distribution studies have shown that MEAP is localized in the lung; in this organ there is very little Met-enkephalin (ME). To test the direct actions of MEAP in a tissue where it is differentially distributed from ME, MEAP was given intratracheally to anesthetized guinea pigs. MEAP produced a dose-related bronchospasm characterized by a slow onset (30-90 sec) and a duration exceeding 5 min. No significant effects on heart rate or blood pressure were observed concomitant with the pulmonary effects. ME or ethylketocyclazocine, tested at the same doses as MEAP , did not produce a bronchospasm. Moreover, naloxone failed to block MEAP 's effects. Therefore, it appears as if the MEAP -induced bronchospasm does not have an opioid-related mechanism of action. The bronchospasm produced by MEAP was not blocked by an anti-histamine (chlorpheniramine) or by vagotomy. However, inhibition of cyclo-oxygenase by ibuprofen blocked the MEAP -induced bronchospasm, suggesting that an arachidonic acid metabolite was involved in the effect. Blockade of MEAP 's pulmonary effects by an inhibitor of thromboxane synthetase (SQ 80338) and not by a leukotriene antagonist (FPL 55712) implied that MEAP 's mechanism of action may be via bronchospastic thromboxanes. These data are the first to demonstrate a non-opioid, thromboxane-mediated action of an enkephalin-related peptide.

UI	MeSH Term	Description	Entries
D007052	Ibuprofen	A non-steroidal anti-inflammatory agent with analgesic, antipyretic, and anti-inflammatory properties	Advil,Benzeneacetic Acid, alpha-methyl-4-(2-methylpropyl)- trimethylsilyl ester,Brufen,Ibumetin,Ibuprofen, (+-)-Isomer,Ibuprofen, (R)-Isomer,Ibuprofen, (S)-Isomer,Ibuprofen, Aluminum Salt,Ibuprofen, Calcium Salt,Ibuprofen, Copper (2+) Salt,Ibuprofen, Magnesium Salt,Ibuprofen, Potassium Salt,Ibuprofen, Sodium Salt,Ibuprofen, Zinc Salt,Ibuprofen-Zinc,Motrin,Nuprin,Rufen,Salprofen,Trauma-Dolgit Gel,alpha-Methyl-4-(2-methylpropyl)benzeneacetic Acid,Ibuprofen Zinc,Trauma Dolgit Gel
D007093	Imidazoles	Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
D008297	Male		Males
D009270	Naloxone	A specific opiate antagonist that has no agonist activity. It is a competitive antagonist at mu, delta, and kappa opioid receptors.	MRZ 2593-Br,MRZ-2593,Nalone,Naloxon Curamed,Naloxon-Ratiopharm,Naloxone Abello,Naloxone Hydrobromide,Naloxone Hydrochloride,Naloxone Hydrochloride Dihydride,Naloxone Hydrochloride, (5 beta,9 alpha,13 alpha,14 alpha)-Isomer,Naloxone, (5 beta,9 alpha,13 alpha,14 alpha)-Isomer,Narcan,Narcanti,Abello, Naloxone,Curamed, Naloxon,Dihydride, Naloxone Hydrochloride,Hydrobromide, Naloxone,Hydrochloride Dihydride, Naloxone,Hydrochloride, Naloxone,MRZ 2593,MRZ 2593 Br,MRZ 2593Br,MRZ2593,Naloxon Ratiopharm
D011312	Pressure	A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Pressures
D001980	Bronchi	The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI.	Primary Bronchi,Primary Bronchus,Secondary Bronchi,Secondary Bronchus,Tertiary Bronchi,Tertiary Bronchus,Bronchi, Primary,Bronchi, Secondary,Bronchi, Tertiary,Bronchus,Bronchus, Primary,Bronchus, Secondary,Bronchus, Tertiary
D002867	Chromones		1,4-Benzopyrones,Chromone,1,4 Benzopyrones
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004744	Enkephalin, Methionine	One of the endogenous pentapeptides with morphine-like activity. It differs from LEU-ENKEPHALIN by the amino acid METHIONINE in position 5. Its first four amino acid sequence is identical to the tetrapeptide sequence at the N-terminal of BETA-ENDORPHIN.	Methionine Enkephalin,5-Methionine Enkephalin,Met(5)-Enkephalin,Met-Enkephalin,5 Methionine Enkephalin,Enkephalin, 5-Methionine,Met Enkephalin
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea