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Plasma branched-chain amino acids and urinary C-peptide-creatinine excretion was determined at 3, 4 1/2 and 6 months of age in a group of 50 infants who were either breast-fed or artificially fed and selected at random. The average concentrations of valine in plasma and C-peptide in urine as well as the ratio between C-peptide and creatinine in urine were 2-3 times higher (p less than 0.01) in artificially fed as compared to breast-fed infants at all the ages studied. Plasma valine values correlated significantly with the urinary C-peptide/creatinine ratio (r = 0.76, p less than 0.01), which suggests that the enhanced insulin response induced by the artificial formula is related to its protein content.
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