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We describe the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. We partly purified the antisera by selective column chromatography. The Bolton-Hunter reagent was used to radioiodinate the isoenzymes. The assay requires 100 microL of serum, includes a solid-phase second-antibody separation, and can be completed in less than 3 h. There was no cross reactivity between the two isoenzymes. As little as 5 micrograms (50 pmol) of each aspartate aminotransferase can be measured per liter of serum.
F Y Leung, and
A E Niblock, and
A R Henderson
January 1971,
Enzyme,
F Y Leung, and
A E Niblock, and
A R Henderson
August 1990,
Clinical biochemistry,
F Y Leung, and
A E Niblock, and
A R Henderson
February 1983,
Clinica chimica acta; international journal of clinical chemistry,
F Y Leung, and
A E Niblock, and
A R Henderson
March 1972,
Quaderni Sclavo di diagnostica clinica e di laboratorio,
F Y Leung, and
A E Niblock, and
A R Henderson
January 1971,
Enzyme,
F Y Leung, and
A E Niblock, and
A R Henderson
January 1987,
Clinical chemistry,
F Y Leung, and
A E Niblock, and
A R Henderson
October 1982,
Equine veterinary journal,
F Y Leung, and
A E Niblock, and
A R Henderson
February 1983,
Clinical chemistry,
F Y Leung, and
A E Niblock, and
A R Henderson
January 1974,
Gigiena truda i professional'nye zabolevaniia,
F Y Leung, and
A E Niblock, and
A R Henderson
January 1984,
Progress in clinical and biological research,
