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Isoelectric focusing (pH 4.0-5.0) of serum alpha 2HS-glycoprotein on polyacrylamide gels has been found to be a useful tool in population genetics and forensic science. Using this method, we isolated three common types, alpha 2HS 1-1, alpha 2HS 2-1 and alpha 2HS 2-2, and showed that alpha 2HS types are determined by two autosomal codominant alleles, alpha 2HS1 and alpha 2HS2. The method is simple, fast and easy to perform. Results of typing for the two alleles, alpha 2HS1 and alpha 2HS2, are described for a Japanese population sample (n = 1003).
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