Biomaterial Database

Measurement of cochlear blood flow--new technique. 1984

J Prazma, and S G Vance, and G Rodgers

A newly-developed procedure which combines the microsphere method with surface preparation dissection was employed in an investigation of blood flow in cochleas of anesthetized gerbils. In these experiments, 10.1 +/- 0.9 microns carbon-coated microspheres were injected into the left auricle of the heart and a reference blood sample withdrawn at 0.165 ml/min from the iliac artery. The blood flow in the lateral portion (stria vascularis, ligamentum spirale, and suprastria) was calculated to be 337.20, 184.00 and 24.75 nl/min; in the spiral portion (basilar membrane, lamina spiralis ossea, and limbus spiralis) it was found to be 85.40, 39.55 and 6.10 nl/min; and in the central portion (modiolus) it was found to be 256.55, 43.60 and 2.45 nl/min in the first, second and third turns, respectively. The total blood flow in the left and right membranous cochleas was 1062.70 and 896.60 nl/min, respectively. Averaged total blood flow in both ears was 979.60 nl/min. This is a first attempt to measure blood flow in the very small, functionally different areas of the cochlea. This work has shown that it is possible to measure and compare blood flow between the turns and between different functional parts within the turns.

UI	MeSH Term	Description	Entries
D008863	Microspheres	Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.	Latex Beads,Latex Particles,Latex Spheres,Microbeads,Bead, Latex,Beads, Latex,Latex Bead,Latex Particle,Latex Sphere,Microbead,Microsphere,Particle, Latex,Particles, Latex,Sphere, Latex,Spheres, Latex
D012039	Regional Blood Flow	The flow of BLOOD through or around an organ or region of the body.	Blood Flow, Regional,Blood Flows, Regional,Flow, Regional Blood,Flows, Regional Blood,Regional Blood Flows
D003051	Cochlea	The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.	Cochleas
D004210	Dissection	The separation and isolation of tissues for surgical purposes, or for the analysis or study of their structures.	Dissections
D005849	Gerbillinae	A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys.	Gerbils,Jird,Meriones,Psammomys,Rats, Sand,Gerbil,Jirds,Merione,Rat, Sand,Sand Rat,Sand Rats
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001489	Basilar Membrane	A basement membrane in the cochlea that supports the hair cells of the ORGAN OF CORTI, consisting keratin-like fibrils. It stretches from the SPIRAL LAMINA to the basilar crest. The movement of fluid in the cochlea, induced by sound, causes displacement of the basilar membrane and subsequent stimulation of the attached hair cells which transform the mechanical signal into neural activity.	Basilar Membranes,Membrane, Basilar,Membranes, Basilar
D013316	Stria Vascularis	A layer of stratified EPITHELIUM forming the endolymphatic border of the cochlear duct at the lateral wall of the cochlea. Stria vascularis contains primarily three cell types (marginal, intermediate, and basal), and capillaries. The marginal cells directly facing the ENDOLYMPH are important in producing ion gradients and endochoclear potential.	Vascularis, Stria