Biomaterial Database

alpha-N-Acetylgalactosaminidase from squid liver: purification and characterization of two enzymes. 1984

T Itoh, and Y Uda

Squid liver contains two kinds of alpha-N-acetylgalactosaminidases, which could be separated by gel filtration on Sephadex G-200 or by SP-Sephadex ion exchange chromatography. The two alpha-N-acetylgalactosaminidases, alpha-N-acetylgalactosaminidase I and II, were purified by procedures involving extraction, ammonium sulfate precipitation, and chromatographies on SP-Sephadex, Sephadex G-100, Sephadex G-200, DEAE-Sephadex, and Sepharose 6B. Enzyme I was purified 1,100-fold and enzyme II 3,000-fold. Both enzymes appeared to be homogeneous based upon the results of disc gel electrophoresis. Enzyme I had a pH optimum of 3.0 and was heat-stable. It was inhibited by N-acetylgalactosamine and galactose. On the other hand, enzyme II had a pH optimum of 4.2 and was heat-labile. Galactose did not affect the enzyme activity. In contrast to enzyme I, which showed alpha-galactosidase activity even in the final preparation, enzyme II was practically free from alpha-galactosidase activity.

UI	MeSH Term	Description	Entries
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D002852	Chromatography, Ion Exchange	Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.	Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D006026	Glycoside Hydrolases	Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules.	Endoglycosidase,Exoglycosidase,Glycohydrolase,Glycosidase,Glycosidases,Glycoside Hydrolase,Endoglycosidases,Exoglycosidases,Glycohydrolases,Hydrolase, Glycoside,Hydrolases, Glycoside
D006596	Hexosaminidases	Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.	Galactosaminidases,Hexosaminidase,Galactosaminidase,Glucosaminidase,Glucosaminidases
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D048809	alpha-N-Acetylgalactosaminidase	A hexosaminidase with specificity for terminal non-reducing N-acetyl-D-galactosamine residues in N-acetyl-alpha-D-galactosaminides.	alpha-N-Acetyl-D-galactosaminidase,N-Acetyl-alpha-D-Galactosaminidase,alpha-D-N-Acetyl-Galactosaminyloligosaccharidase,alpha-D-N-Acetylgalactosaminyloligosaccharidase,alpha-Galactosidase B,endo-N-Acetyl-alpha-D-Galactosamidase,endo-alpha-N-Acetylgalactosaminidase,N Acetyl alpha D Galactosaminidase,alpha D N Acetyl Galactosaminyloligosaccharidase,alpha D N Acetylgalactosaminyloligosaccharidase,alpha Galactosidase B,alpha N Acetyl D galactosaminidase,alpha N Acetylgalactosaminidase,endo N Acetyl alpha D Galactosamidase,endo alpha N Acetylgalactosaminidase