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An exchange assay is described for the measurement of nuclear estrogen receptors (ERn) in microdissected brain regions. The distribution of ERn in the hypothalamus and amygdala of the rat 1 h after an injection of estradiol (E) is presented. Combining the exchange assay with a previously described method for measurement of cytosol estrogen receptors (ERc) in microdissected brain samples, gonadectomized male and female rats were compared for ERc and ERn. While ERc concentrations tended to be higher in females than in males in all regions of the hypothalamus, with a significant sex difference in the arcuate-median eminence, no sex difference in ERn concentrations was observed after E injection. These results suggest that ERc measurements alone are not sufficient to establish the capacity of the E receptor system: ERn measurements are also necessary to establish the relationship between receptor levels and physiologic estrogen responsiveness.
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
November 1977,
Steroids,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
June 1994,
Molecular and cellular neurosciences,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
December 1991,
Physiology & behavior,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
December 1989,
Neuroendocrinology,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
January 1975,
Methods in enzymology,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
May 1989,
Neuroendocrinology,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
December 1969,
Biochimica et biophysica acta,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
March 1991,
Endocrinology,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
October 1975,
Brain research,
J D Kranzler, and
E E Jones, and
N J MacLusky, and
H Sakamoto, and
F Naftolin
October 1982,
Gan,
