-transparent.png){kind=logo}
Muscarinic cholinergic receptor sites in human gastric mucosa were analyzed directly by using radioligand binding techniques with the specific muscarinic antagonist 3H-quinuclidinyl benzilate (QNB) as ligand. Specific binding of 3H-QNB to membrane preparations from human gastric mucosa was saturable, of high affinity (Kd = 4.17 +/- 1.94 nM, Bmax = 0.37 +/- 0.04 pmol/mg protein) and selectively inhibited by muscarinic antagonists (atropine, scopolamine) and agonists (acetylcholine, pilocarpine). These findings provide direct evidence for the existence of muscarinic cholinergic receptors in human gastric mucosa. The specific 3H-QNB binding to its receptor was blocked by atropine but not by histamine, cimetidine, pentagastrin, or synthetic human gastrin. The muscarine and histamine H2-receptor, or muscarine and gastrin receptor, probably do not share the same locus.
T Tokunaga, and
R Nishimura, and
M Akagi
January 1984,
European journal of respiratory diseases. Supplement,
T Tokunaga, and
R Nishimura, and
M Akagi
December 1990,
Digestive diseases and sciences,
T Tokunaga, and
R Nishimura, and
M Akagi
May 1997,
Plant molecular biology,
T Tokunaga, and
R Nishimura, and
M Akagi
March 1978,
Nature,
T Tokunaga, and
R Nishimura, and
M Akagi
January 1987,
ORL; journal for oto-rhino-laryngology and its related specialties,
T Tokunaga, and
R Nishimura, and
M Akagi
August 2008,
European urology,
T Tokunaga, and
R Nishimura, and
M Akagi
July 2009,
Ginecologia y obstetricia de Mexico,
T Tokunaga, and
R Nishimura, and
M Akagi
November 1998,
Neurology,
T Tokunaga, and
R Nishimura, and
M Akagi
March 2000,
Pharmaceutica acta Helvetiae,
T Tokunaga, and
R Nishimura, and
M Akagi
June 1995,
Gut,
