Biomaterial Database

[A comparison of methods for the enrichment of Salmonellae from human feces]. 1982

H E Müller

154 samples of faeces contaminated with salmonellae belonging to 21 species were investigated each in 23 enrichment broths for compare the efficiency. The best results were obtained with selenite cystine broth (no. 5) followed by Rappaport broth (no. 17) and tetrathionate brilliant green bile broth - TBG - (no. 12); see also Fig. 1-4 and Tables 3 and 4. S. typhi is recovered the best by selenite broths. Rappaport broth seems to be the optimal enrichment broth for the most salmonellae with exception of S. typhi, S. bovismorbificans, and some other species. Preuss's modification of tetrathionate enrichment broth was significantly more efficient than Muller-Kauffmann's tetrathionate. Brilliant green bile broths are not very selective. Its efficiency is comparable with unselective broths without any inhibiting substances. The unspecific broths show a little enrichment effect during short incubation times. Therefore, they are used for preenrichment. However, incubation during 24 h is recommended for optimal recovery in selective enrichment broths, i.e. Rappaport, selenite, and tetrathionate media. The selenite broth should be used unconditionally for detecting salmonellae in human faeces. It should be combined additionally with Rappaport or tetrathionate broth in the modification of Preuss but not of Muller-Kauffmann.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012482	Salmonella paratyphi A	A serotype of SALMONELLA ENTERICA that causes mild PARATYPHOID FEVER in humans.	Salmonella paratyphi,Salmonella enterica serovar Paratyphi A
D012484	Salmonella paratyphi B	A serotype of SALMONELLA ENTERICA which is an agent of PARATYPHOID FEVER in humans.	Salmonella schottmuelleri,Salmonella enterica serovar Paratyphi B
D012486	Salmonella typhimurium	A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.	Salmonella typhimurium LT2
D012643	Selenium	An element with the atomic symbol Se, atomic number 34, and atomic weight 78.97. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.	Selenium-80,Selenium 80
D013776	Tetrathionic Acid	A sulfuric acid dimer, formed by disulfide linkage. This compound has been used to prolong coagulation time and as an antidote in cyanide poisoning.	Sodium Tetrathionate,Potassium Tetrathionate,Tetrathionic Acid, Calcium Salt (1:1),Tetrathionic Acid, Dipotassium Salt,Tetrathionic Acid, Disodium Salt,Tetrathionic Acid, Disodium Salt, Dihydrate,Tetrathionate, Potassium,Tetrathionate, Sodium