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Complete purification of human renin from noncancerous, autopsied kidneys is reported. A 480,000-fold purification was achieved to yield renin with a specific activity of 950 Goldblatt units/mg. This preparation satisfied multiple criteria of purity as tested by polyacrylamide gel electrophoresis, isoelectric focusing, specific activity, analytical ultracentrifugation, and immunodouble diffusion. The molecular weight of the pure enzyme determined by sedimentation equilibrium is 40,000. The apparent molecular weight estimated by gel filtration is 41,000. The enzyme has an isoelectric point of pH 5.7. Human renin shows an affinity for concanavalin A, suggesting the presence of carbohydrates. These properties and the amino acid composition of human renin are different from those of renin obtained from other mammalian species. Human renin antibodies prepared with the pure enzyme preparation showed negligible cross-reactivity with renin from other mammalian species. The activity with homologous human renin substrate has a pH optimum of 6, whereas with substrates from other mammalian species the optima were in higher or lower pH ranges.
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
November 1979,
Biochemistry,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
September 1986,
Biochemical and biophysical research communications,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
December 1978,
Clinical science and molecular medicine. Supplement,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
January 1986,
Journal of biochemistry,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
June 1979,
The Journal of biological chemistry,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
April 1988,
Biochimica et biophysica acta,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
October 1978,
Biochimica et biophysica acta,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
October 1970,
The Journal of laboratory and clinical medicine,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
August 1966,
Biochimica et biophysica acta,
H Yokosawa, and
L A Holladay, and
T Inagami, and
E Haas, and
K Murakami
July 1978,
Biochemical and biophysical research communications,
