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The Furth murine mastocytoma was adapted to the ascitic form and separated into fractions enriched with respect to lymphocytes and malignant cells by velocity sedimentation in the SZ-14 reorienting zonal rotor or in the isokinetic gradient. Lymphocytes were more highly purified (p less than 0.01) in the isokinetic gradient than in the zonal rotor, i.e., lymphocytes comprised 99.1% of the nucleated cells in the purest fraction from the isokinetic gradient and 80.1% of the nucleated cells in the purest fraction from the zonal rotor. Neoplastic mast cells were similarly purified by the two methods; they comprised 67.7 and 78.5% of the nucleated cells in the purest fractions from the isokinetic gradient and zonal rotor, respectively. Up to 160 million tumor cells can be purified in a single step with the reorienting zonal rotor, whereas 30 to 40 million cells per gradient approach the limit of the isokinetic gradient. After centrifugation in the zonal rotor, recovery was 85.6 +/- 12% (S.D.) of the cells layered over the gradient; and the separated tumor cells retained their ability to form tumors when transplanted into mice. The separation of large numbers of lymphocytes and malignant cells from the same tumor in the SZ-14 rotor should aid in the biochemical and immunological characterization of cancer.
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
November 1980,
Clinical and experimental immunology,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
October 1969,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
February 1977,
Cancer research,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
April 1975,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
March 1972,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
January 1971,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
May 1981,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
July 1973,
Analytical biochemistry,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
August 1979,
Neurochemical research,
C L Green, and
T P Pretlow, and
K A Tucker, and
E L Bradley, and
W J Cook, and
A M Pitts, and
T G Pretlow
June 1970,
Journal of the National Cancer Institute,
